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Goal: You will be given a sample of a solution of bovine heart cytochrome c. You will use the Coomassie® Plus Protein Assay from Pierce to determine the concentration of protein in the sample.Part of MIT OpenCourseWare (http://ocw.mit.edu) course "Chemistry Lab Techniques", these labs were designed to give first year MIT students practical hands-on introduction to the basic techniques of experimental chemistry in a laboratory setting. Labs bring science concepts alive as students observe, practice, and experiment with problems. High school science teachers and advanced high school students will find these materials useful. The MIT site has additional related videos and readings. Sarah A. Tabacco, Chemistry Lab Techniques, Fall 2004. (Massachusetts Institute of Technology: MIT OpenCouseWare), http://ocw.mit.edu (Accessed July 2008). License: Creative Commons BY-NC-SA http://ocw.mit.edu/OcwWeb/web/terms/terms/index.htm#cc http://creativecommons.org/licenses/by-nc-sa/3.0/us/legalcode WARNING NOTICE The experiments described in these materials are potentially hazardous and require a high level of safety training, special facilities and equipment, and supervision by appropriate individuals. You bear the sole responsibility, liability, and risk for the implementation of such safety procedures and measures. MIT shall have no responsibility, liability, or risk for the content or implementation of any of the material presented. Legal Notice
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