Type:

Other

Description:

This lab describes two biochemical assays: One for measuring acetylcholinesterase activity and one for measuring protein concentration. Students learn how to manipulate small-volume samples, use a standard spectrophotometer or a microplate reader spectrophotometer, construct a standard curve, and normalize data. The lab is intended to be used in conjunction with a cell culture lab in which PC12 cells are exposed to various agents that influence their phenotypic state.

Subjects:

    Education Levels:

      Keywords:

      signal transduction,NSDL,NSDL_SetSpec_BEN,neuron,laboratory exercise,cell culture,enzyme,phenotype,Life Science,spectrophotometer,chromaffin cell,oai:nsdl.org:2200/20080618221454960T

      Language:

      English

      Access Privileges:

      Public - Available to anyone

      License Deed:

      Creative Commons Attribution Non-Commercial Share Alike

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