Type:

Other

Description:

These manuals are used in the Stem Cell Culture Course at City College of San Francisco. This course is about general mammalian cell culture techniques but includes a laboratory exercise using stem cells (takes 3 weeks to complete). The course is taught to high school students but the materials are also used for college students. Laboratory exercises provide instruction in basic techniques of routine cell culture using common cell lines before progressing to differentiation of mouse embryonic stem cells. Photographs and explanations of common equipment (laminar flow hood, inverted microscope, etc.) and reagents are provided. Laboratory exercises include the following: Basic Aseptic Technique; Media Preparation; Plating cells from frozen stock; Cell counting and plating; Survival assay (UV); Live Cell Identification; Transfection; Freezing cells; Stem cell differentiation. A student lab manual and an instructor manual are provided.

Subjects:

  • Science > Chemistry
  • Science > Biology
  • Science > Technology
  • Education > General

Education Levels:

  • Grade 1
  • Grade 2
  • Grade 3
  • Grade 4
  • Grade 5
  • Grade 6
  • Grade 7
  • Grade 8
  • Grade 9
  • Grade 10
  • Grade 11
  • Grade 12

Keywords:

Informal Education,NSDL_SetSpec_ncs-NSDL-COLLECTION-000-003-112-021,Chemistry,NSDL,High School,Technical Education (Lower Division),Undergraduate (Lower Division),oai:nsdl.org:2200/20120406113330562T,Undergraduate (Upper Division),Vocational Education -- Technical,General Public,Technical Education (Upper Division),Higher Education,Education,Vocational/Professional Development Education,Life Science,Science -- Biology,Technology,Science -- Chemistry,Science -- Technology

Language:

English

Access Privileges:

Public - Available to anyone

License Deed:

Creative Commons Attribution Non-Commercial Share Alike

Collections:

None
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