Type:

Other

Description:

A modular patch-clamp amplifier was constructed based on the Strickholm design, which was initially published in 1995. Various parts of the amplifier such as the power supply, input circuit, headstage, feedback circuit, output and nulling circuits were redesigned to use recent software advances and fabricated using the common lithographic printed circuit board fabrication process and commercially available electronic components. The calibration, validation, and regular recording procedures along with the results of an actual recording of inward Ca2+ currents from PC12 neuronal cells are described in detail. This work describes the construction of a low-cost patch-clamp amplifier and setting up an electrophysiology recording system in a laboratory with regular technical expertise. The constructed amplifier provides an inexpensive yet practical tool for research and teaching purposes while the experience obtained during construction and setting up of the patch-clamp amplifier provides the basic and advanced understanding required for operating an advanced cell potential recording apparatus.

Subjects:

  • Education > General

Education Levels:

    Keywords:

    Education,NSDL,NSDL_SetSpec_BEN,Career,oai:nsdl.org:2200/20110607195108943T,Electrophysiology,Life Science,Teacher-centered/traditional instruction,Tutorial or self-directed instruction,Physiology,Student-centered instruction

    Language:

    English

    Access Privileges:

    Public - Available to anyone

    License Deed:

    Creative Commons Attribution Non-Commercial Share Alike

    Collections:

    None
    This resource has not yet been aligned.
    Curriki Rating
    'NR' - This resource has not been rated
    NR
    'NR' - This resource has not been rated

    This resource has not yet been reviewed.

    Not Rated Yet.

    Non-profit Tax ID # 203478467