Type:

Other

Description:

Teaching the practical aspects of signal transduction to large undergraduate classes can be challenging when there is only a finite time frame in which to engage in laboratory activities. This teaching resource describes the use of bead-based ALPHAscreen technology for a class of 300 second-year biochemistry students, exposing the next generation of researchers to cutting-edge technology. Although in this case phosphorylated extracellular signal–regulated kinase 1 and 2 were measured, this technology is applicable to the measurement of many different signaling components. This resource provides a practical guide for instructors and exemplifies how such traditionally high-throughput research technologies can be used as teaching tools.

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      Keywords:

      oai:nsdl.org:2200/20100927005213836T,protocol,NSDL,NSDL_SetSpec_BEN,biochemical assay,signal transduction,cell culture,G protein-coupled receptor (GPCR),extracellular signal-regulated kinase (ERK),Life Science,drug discovery,History/Policy/Law,protein detection

      Language:

      English

      Access Privileges:

      Public - Available to anyone

      License Deed:

      Creative Commons Attribution Non-Commercial Share Alike

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