Type:

Other

Description:

This item is a comprehensive tutorial on TIRFM (total internal reflection fluorescence microscopy). The technology, developed in the 1980's, is used extensively in cell and molecular biology for observation of thin regions of a specimen, often less than 200nm. This tutorial includes fundamental concepts, theoretical aspects, basic and advanced microscope configurations, and a set of Java applets that depict the fluorophore excitation process and instrument configurations.

Subjects:

  • Education > General
  • Mathematics > General

Education Levels:

  • Grade 1
  • Grade 6
  • Grade 8
  • Grade 9

Keywords:

NSDL,Undergraduate (Lower Division),evanescent field,Mathematics,fluorophore,TIRF,Physics,virtual microscopes,Life Science,Informal Education,Higher Education,Geometrical Optics,tutorials,oai:nsdl.org:2200/20080905115739826T,Optics,optics,total internal reflection fluorescence,microscopy,Engineering,NSDL_SetSpec_439869,fluorescence microscopy,Vocational/Professional Development Education,Simulations,Education,Technology

Language:

English

Access Privileges:

Public - Available to anyone

License Deed:

Creative Commons Attribution Non-Commercial Share Alike

Collections:

None
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