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This laboratory activity, by the Biotechnology Education and Training Sequence Investment (BETSI) project at Southwestern College, walks students and educators through the procedure of restriction enzyme mapping. Restriction enzymes are found in bacteria and "cleave the double helix of DNA at specific places." This activity, which is broken up into two parts, allows students to observe this process by presenting specific procedure steps and illustrations to guide them. There is also a worksheet for students to complete: Analysis of a Restriction Enzyme Digest of a Plasmid. This is a helpful activity for any biotechnology classroom to give students hands-on experiences with enzymes and the reconstruction of recombinant DNA molecules.
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