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Winter dormant organisms, both plants and animals, have two general categories of adaptation for survival of exposure to cold climate stress. They can resist the formation of internal ice by supercooling through the production of antifreeze compounds; or, they can tolerate internal ice by addition of cryoprotectant biochemicals to their body fluids. In the latter case, nucleator chemicals may be produced to promote the formation of ice in extracellular fluids. We will use techniques to measure supercooling points of a winter dormant animal, the goldenrod gallfly, and to evaluate its seasonal production of cryoprotectant chemicals.
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